Summarized by Masters of Longevity from Nature - Biotechnology.
Describes a prototype closed-system platform that integrates multilayer culture plates and a G-Rex vessel to enable iPSC establishment, expansion, and differentiation under closed conditions.
Key Takeaways
- Seeding 1 × 10^6 cells produced 1.64 × 10^5 adherent cells across 19 plates, a 16.4% adhesion rate.
- TRA-1-60 positive iPSC colonies appeared by day 19, averaging 0.79 ± 1.17 colonies per plate.
- IPSCs cultured on thermo-responsive Smart Surface Culture Ware retained OCT3/4 >93% and showed no detectable karyotypic abnormalities across five passages.
