A methods-focused article describing a virus-like particle toolkit for delivering diverse CRISPR edits and enabling pooled functional genomics in primary human myeloid cells.
Key Takeaways
- VLP-mediated delivery of ribonucleoproteins supports gene knockout, base editing and epigenetic silencing
- Combined with adeno-associated virus-mediated donor delivery
- Which pairs sgRNA delivery by VPX-lentivirus with Cas9 protein delivery by engineered VLPs