Summarized by Masters of Longevity from PR Newswire.
Reports a methodological advance that improves in vitro circular RNA production by combining target-site selection with engineering of a self-targeting P1 construct.
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Key Takeaways
- Engineering a P1 construct with a short polyA10 and antisense sequence increased self-circularization up to about seven-fold.
- Optimized STS circularization achieved roughly two-fold higher efficiency for ~7.8 kb Factor VIII RNA versus the PIE method.
- Self-circularization efficiency varied significantly by target-site location even within the same RNA sequence.