Summarized by Masters of Longevity from Nature - Biotechnology.
Review of emerging enzyme platforms and engineering strategies that enable targeted kilobase-scale DNA insertions without double-strand breaks.
Key Takeaways
- Although these approaches enable precise small-scale sequence changes, methods for programmable insertion of large DNA cargos have been limited.
- The ability to write entire genes or large regions into the genome could transform the treatment of genetically heterogeneous disorders, for which numerous pathogenic variants underlie a common disease and mutation-specific editing strategies are impractical.
- Recent advances in computational genome mining have accelerated the discovery of naturally occurring enzymes with novel biochemical and functional properties, including recombinases and transposases capable of large-scale modifications.
