Reports multi‑year human cerebral organoid cultures that continue cell‑type specific maturation and record elapsed time via transcriptional and epigenomic changes.
Key Takeaways
- Human cerebral organoids were cultured and profiled over five years to track long‑term maturation
- Whole‑genome methylation profiles of organoids correlated precisely with time spent in vitro
- Older neural progenitors in chimeric organoids produced late neuronal fates and skipped earlier progeny