A methods-focused research article examining how a precise DNA quantification technique maps transgene copy variation to disease trajectory in an ALS mouse model.
Key Takeaways
- Droplet digital PCR (ddPCR) quantified SOD1 G93A transgene copies between 51.68 and 119.5 copies per mouse
- Real-time qPCR reported lower copy ranges of 23 to 44.65 and still identified genotype reliably
- DdPCR copy number correlated significantly with disease phenotype and lifespan in SOD1 G93A mice