Summarized by Masters of Longevity from Nature - Biotechnology.
Report on engineered ionizable lipids that switch charge with pH to create lipid nanoparticles which deliver mRNA and DNA efficiently while reducing inflammatory toxicity in cells and animals.
Key Takeaways
- A library of 144 switchable lipids produced SNPs that are negatively charged at pH 7.4 and neutralize between pH 4.2 and 5.8.
- SNPs delivered mRNA and plasmid DNA to mice and human PBMCs as efficiently as traditional LNPs but did not trigger cytokine production.
- SNPs carrying therapeutic mRNA avoided acute toxicity in mice and improved treatment of LPS-induced acute lung injury compared with traditional LNPs.
