Summarized by Masters of Longevity from Nature - Biotechnology.
A research article describing a genetically engineered pig model used to study activation strategies for a human-relevant heart disease mutation, with comparisons of reproductive and postnatal activation methods.
Key Takeaways
- A double-cassette inducible humanized phospholamban R14del allele was engineered into pigs to model dominant cardiomyopathy.
- Cre-mediated activation delivered via treated donor cells or Cre mRNA zygote microinjection produced piglets with the activated R14del mutation.
- Postnatal delivery of Cre using adeno-associated virus also activated the mutation in piglets but with variable efficiency.
