A lab study examining how ultrafast 400 nm laser pulses affect MRSA biofilms, combining molecular, metabolic, and imaging analyses to assess prevention and eradication.
Key Takeaways
- Femtosecond laser at 400 nm reduced biofilm viability by 75.3% using optimized exposure parameters
- Laser treatment decreased biomass by 44.5% and cut metabolic activity by 60–85% after 45 minutes
- RT-qPCR showed broad transcriptional dysregulation of quorum sensing genes linked to biofilm attenuation