Protein-aging research that walks you through how scientists evolved an enzyme to enzymatically reverse a common age-related protein modification, and why this could open paths for molecular repair in tissues.
Key Takeaways
- Researchers engineered a CML-cleaving enzyme through directed evolution of over 500 million variants
- Engineered enzyme restores native lysine by oxidizing Nε-carboxymethyl-lysine on peptides and proteins
- Activity was demonstrated on model proteins in vitro and on human tissue samples from elderly donors