How cancer researchers track changes in extrachromosomal DNA behavior during drug exposure, with methods and implications for adaptive resistance.
Key Takeaways
- Single-cell DNA sequencing with cellular barcoding was used to track ecDNA segregation across clones
- Some clones change their ecDNA segregation mode under drug treatment rather than only being selected
- Active, nonrandom regulation of ecDNA segregation can generate drug-resistant cell populations