Summarized by Masters of Longevity from Nature - Biotechnology.
NanoLC-TIMS-TOF lipidomics methods for profiling very small plasma volumes, comparing microscale extraction approaches and buffer conditions to guide sample-limited workflows.
Key Takeaways
- Positive-ion nanoLC-TIMS-TOF can profile broad lipid classes from as little as 1 µL of plasma while retaining dominant annotated lipid classes.
- Single-phase microscale extractions show extraction- and volume-dependent differences in detected features, so profiling depth declines with lower plasma input.
- Using 5 mM ammonium formate in the mobile phase balanced maximal feature detection with stable chromatographic pressure during extended runs.
