Describes an mRNA delivery workflow enabling transient fluorescent reporters to monitor metabolism and cellular stress across primary and stem cells.
Key Takeaways
- IVT-synthesized reporter mRNAs achieved high transfection efficiencies in primary fibroblasts and iPSCs
- Three ratiometric reporters detected subcellular pH, hydrogen peroxide, and ATP levels effectively
- MRNA delivery avoided genome integration and bypassed stable cell line generation requirements