A step-by-step protocol describes combining prime editing and recombinase insertions into repetitive elements to enable massively multiplexed, programmable genome rearrangements and fitness mapping.
Key Takeaways
- Yet existing methods are limited by the low density of achievable modifications or the toxicity of methods
- This scale of genome editing has not previously been attainable and supports a wide range of studies
- Full protocol takes ~12–18 weeks to complete and requires intermediate to advanced expertise in genome editing