Report on how low-oxygen tumor environments boost adenovirus replication via a conserved hypoxia-responsive promoter element, with mechanistic and in vivo context.
Key Takeaways
- Hypoxia increased infectious adenovirus particle yield 4- to 14-fold in multiple cancer cell lines
- HRE in the viral E1A promoter drove a 17-fold E1A upregulation within two hours under hypoxia
- HIF-2α and HIF-1β heterodimers bound the E1A HRE and transactivated viral gene expression