Discusses reprogramming guide RNAs to reduce off-target effects and expand CRISPR utility for mRNA sensing, multiplexed editing, and safer tools.
Key Takeaways
- CrRNA:tracrRNA duplex in guide RNAs can be physically split and sequence-reprogrammed
- Endogenous transcripts containing crRNA-like sequences can hijack gRNAs
- Machine-learning-derived design rules produced crRNA variants mismatched to human and mouse transcriptomes