Describes a step-by-step protocol to inoculate stirred suspension bioreactors directly with cryopreserved human pluripotent stem cells for scalable production.
Key Takeaways
- Strategies for hPS cell production in stirred-tank bioreactors (STBR)
- Resulting cells can be used for the generation of high-density cryostocks
- Key feature of this Protocol is the nonenzymatic