CRISPR/Cas9 tool design and plasmid transfection in a fish muscle cell line, showing how researchers developed and tested gene‑editing constructs.
Key Takeaways
- SgRNAs targeting exon 1 of the mstnb gene were cloned into a Cas9-GFP plasmid and sequence-verified
- Transfection produced distinct GFP-positive LRDM muscle cells at passages 10, 20, and 30
- Study established functional CRISPR/Cas9 constructs for mstnb in Labeo rohita muscle cells