Report on comparing two delivery strategies for synthetic oligonucleotides to suppress a soil fungal pathogen via gene silencing and their relative efficacy.
Key Takeaways
- Whey protein concentrate microencapsulation produced stable spherical microcapsules about 0.2–0.36 µm in diameter
- Both methylated and WPC-encapsulated oligoDNAs reduced target gene expression
- Fluorescence microscopy associated Cy3-labeled oligoDNA with fungal hyphae but could not confirm intracellular uptake