CD25 knockout lymphoid cell–line methods and assay setup are presented, offering a practical model to study IL-2 beta/gamma receptor–specific stimulation.
Key Takeaways
- CRISPR/Cas9 was used to generate stable CD25 knockout CTLL-2 and Kit225 lymphoid cell lines
- CD25-KO lines enabled proliferation and STAT5 phosphorylation assays specific to IL-2 beta/gamma signaling
- Panel of IL-2-derived muteins including agonists, super-agonists and antagonists was characterized using these models