An orthogonal CRISPR-activated promoter platform is presented that decouples transcription to enable tunable, predictable multi-gene expression control in yeast.
Key Takeaways
- CAPO platform uses synthetic CRISPR-activated promoters activated only by matching gRNAs and dCas9-VPR
- Tuning guide RNA abundance with defined T7 promoters produced expression comparable to strong native yeast promoters
- CAPO was applied to program fluorescence outputs and to optimize lycopene and 3-hydroxypropionic acid biosynthesis