Summarized by Masters of Longevity from Nature - Biotechnology.
A new benchtop proximity ligation assay, SPLASH, enables ultrasensitive plasma protein quantification for Alzheimer’s biomarkers using standard qPCR equipment.
Key Takeaways
- SPLASH achieved sub-pg/mL limits of detection for five AD biomarkers, ranging from 0.0005 to 0.119 pg/mL.
- Plasma pTau-217 measured by SPLASH discriminated amyloid-positive from amyloid-negative individuals with AUC = 0.839.
- SPLASH is compatible with dried plasma spot samples enabling decentralized collection without cold-chain storage.
