Summarized by Masters of Longevity from Nature - Biotechnology.
A methods-focused article presenting a paper-based 3D cell culture platform for measuring cholesterol efflux capacity, useful for researchers seeking alternative assay formats.
Key Takeaways
- Foam cell formation was induced by PMA-driven macrophage differentiation followed by BODIPY-cholesterol loading and was confirmed by microscopy and CD marker imaging.
- Cholesterol efflux capacity measured from the paper-based platform correlates strongly with results from a commercial cell-based CEC assay across volunteer samples.
