Summarized by Masters of Longevity from Nature - Protocols.
A methodological article outlining a high-throughput, iPSC-based drug screening workflow to find and triage candidate antifibrotic compounds.
Key Takeaways
- The platform uses human reporter iPSC-derived cardiac fibroblasts for high-throughput phenotypic screening of antifibrotic candidates.
- The workflow can screen roughly 5,000 compounds within six to eight weeks using a 384-well plate quantitative HTS format.
- Counter-screening with iPSC-derived cardiomyocytes is incorporated to exclude compounds with potential cardiotoxicity.
