Summarized by Masters of Longevity from Nature - Protocols.
Single-cell DNA methylation protocol article outlining a scalable lab and analysis workflow for parallel methylome profiling of many individual cells.
Key Takeaways
- DNA methylation is a fundamental epigenetic modification that regulates gene expression, maintains genomic integrity and defines cellular identity.
- Here, we present multiplexed single-cell RRBS (Msc-RRBS), a streamlined and scalable protocol for parallel DNA methylation profiling of hundreds of individual cells.
- All enzymatic reactions, including cell lysis, restriction digestion, end repair and adapter ligation, are carried out in a single microwell, which eliminates the need for buffer exchange and minimizes DNA loss.
