Trimbody with rigid AI-designed scaffolds enables atomic-resolution cryo-EM structure determination of small proteins
Designed scaffolds to enable atomic-resolution cryo-EM of small (<50 kDa) proteins. The method stabilizes nanobody–scaffold complexes and produced 2.3–2.6 Å maps for four test proteins, with coordinates deposited in the PDB.
Why it matters2.3-2.6 Å cryo-EM maps for four sub-50 kDa proteins enabled precise PDB model deposition, accelerating structure-guided drug design.