Synthesis, characterization, antioxidant and anticancer potential of Kalanchoe pinnata green synthesized silver nanoparticles targeting p53/MDM2 nexus in hepatic cancer: integrated in vitro and in silico study
The study describes green synthesis of silver nanoparticles (AgNPs) using Kalanchoe pinnata ethanolic leaf extract and characterizes them by DLS, TEM and FTIR, reporting an average hydrodynamic radius of ~4.74 nm and a zeta potential of +23.91 mV. In vitro HepG2 assays and in silico analysis showed increased ROS, apoptosis, and reduced cell viability, with suggested engagement of the p53/MDM2 nexus; GC–MS identified 33 bioactive compounds including β-amyrin.
Why it mattersKp-AgNPs from Kalanchoe pinnata induced ROS-mediated apoptosis in HepG2 cells, enabling p53/MDM2-targeted HCC therapy development.