Spatial perturb-seq: single-cell functional genomics within intact tissue architecture
Spatial Perturb-Seq is an in vivo pooled CRISPR screening platform that integrates perturbations with spatially resolved single-cell readouts in intact mouse brain tissue. Applied to neurodegeneration risk genes, the method uncovers cell-autonomous and microenvironmental effects on neuronal communication and provides open-source code for rapid adoption.
Why it mattersSpatial Perturb-Seq in mouse brain identifies neurodegeneration gene effects, guiding preclinical target prioritization decisions.