Molecular tension indicators reveal unexpectedly complex regulation of tension in live mouse organs
Researchers created single‑fluorophore molecular tension indicators (circularly permuted EGFP with elastic linker and mCherry) inserted into α‑actinin or α‑catenin and established H11 knock‑in reporter mice, enabling superresolution visualization of molecular‑specific and heterogeneous mechanical load regulation across live mouse organs; plasmids deposited to Addgene.
Why it mattersH11 knock-in mice expressing cpEGFP tension indicators enable tissue-level mechanobiology assays, guiding preclinical target prioritization.