Evaluation of phenolic profile and multi-biological activities of Lepista glaucocana extracts optimized by ANN-GA and RSM models
Study compares RSM and ANN-GA extraction optimization for Lepista glaucocana, finding ANN-GA yields extracts with higher antioxidant, anticholinesterase, and dose-dependent antiproliferative activity alongside elevated phenolic levels (gallic, protocatechuic, syringic acids) by LC-MS/MS, suggesting the mushroom as a potential natural source of multifunctional bioactives for further neuroprotective and anticancer lead development.
Why it mattersANN-GA extract with higher gallic acid and AChE inhibition indicates prioritizing LC‑MS-guided fractionation for neuroprotective leads.