Engineered Un1Cas12f1 for multiplex genome editing with enhanced activity and targeting scope
Engineered evoCas12f — a five‑mutation Un1Cas12f1 variant — expands PAM recognition to NTNR/NYTR and increases editing efficiency up to 91%. It enables AAV-compatible multiplexed editing, transcriptional activation, precise base editing, and produced homozygous F0 mice.
Why it mattersAAV-delivered evoCas12f expands PAM range and boosts editing, so preclinical programs should redesign target loci and payloads.