CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization
CRAGE-RB-PI-seq combines recombinase-assisted genome engineering and barcoded promoter insertion sequencing to amplify bacterial transcripts during Arabidopsis root colonization, mapping thousands of promoter activities in Pseudomonas simiae WCS417 and identifying immune-evasion genes; sequencing deposited as PRJNA1221951.
Why it mattersCRAGE-RB-PI-seq enables selection of actionable rhizobacterial targets like xanthine dehydrogenase for engineering.