Benchmarking EGF signaling pathway inference using phosphoproteomics and kinase-substrate interactions
Study benchmarks methods to infer EGF signaling pathways from phosphoproteomics and kinase–substrate networks. Authors assembled three comprehensive EGF-response datasets, found literature-curated networks best recovered ground-truth, and report up to 90% of inferred interactions are absent from current reference sets.
Why it mattersPXD056666 phosphoproteomics data show 90% of kinase interactions missing from ground truth.